通过人类蓝色视觉色素衍生转基因的表达所确定，蓝色视锥细胞和视锥双极细胞具有转录特异性。
Blue cones and cone bipolar cells share transcriptional specificity as determined by expression of human blue visual pigment-derived transgenes.
作者信息
Chiu M I, Nathans J
机构信息
Department of Molecular Biology and Genetics, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
出版信息
J Neurosci. 1994 Jun;14(6):3426-36. doi: 10.1523/JNEUROSCI.14-06-03426.1994.
Sequences 5' of the human blue visual pigment gene have been assayed in transgenic mice for their ability to direct cell-type-specific expression of linked beta-galactosidase (lacZ) and placental alkaline phosphatase (P ALP1) reporters. Constructs containing either 5.4 kilobases (kb) or 0.47 kb of 5' flanking DNA direct expression exclusively to the retina. Within the retina, transgene expression is confined to blue cones and cone bipolar cells, as determined, respectively, by double labeling with anti-cone pigment antibodies and by morphologic analyses. These results imply that blue cones and cone bipolar cells have partially overlapping transcriptional specificities.
已在转基因小鼠中检测了人类蓝色视觉色素基因5'端序列指导连接的β-半乳糖苷酶（lacZ）和胎盘碱性磷酸酶（PALP1）报告基因进行细胞类型特异性表达的能力。含有5.4千碱基（kb）或0.47 kb 5'侧翼DNA的构建体仅将表达导向视网膜。在视网膜内，通过用抗视锥色素抗体进行双重标记以及形态学分析分别确定，转基因表达局限于蓝色视锥细胞和视锥双极细胞。这些结果表明蓝色视锥细胞和视锥双极细胞具有部分重叠的转录特异性。
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