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从真菌生物质中提取和分析吲哚衍生物。

Extraction and analysis of indole derivatives from fungal biomass.

作者信息

Gartz J

机构信息

Department of Fungal Biotransformation, KAI e. V./WIP, Leipzig, Germany.

出版信息

J Basic Microbiol. 1994;34(1):17-22. doi: 10.1002/jobm.3620340104.

DOI:10.1002/jobm.3620340104
PMID:8207663
Abstract

The occurrence and extraction of indole derivatives in six species from four genera of higher fungi were investigated. By using pure methanol for extraction of the mushrooms analysis revealed the highest concentrations of psilocybin and baeocystin. The psilocin content of the species was higher by using aqueous solutions of alcohols than with methanol alone but was an artificial phenomenon caused by enzymatic destruction of psilocybin. The extraction with dilute acetic acid yielded better results than with the water containing alcohols. The simple one-step procedure with methanol for the quantitative extraction is still the safest method to obtain the genuine alkaloids from fungal biomass.

摘要

对四个属的六种高等真菌中吲哚衍生物的产生和提取进行了研究。通过使用纯甲醇提取蘑菇,分析显示了最高浓度的裸盖菇素和脱磷酸裸盖菇素。与单独使用甲醇相比,使用醇类水溶液提取该物种中的裸盖菇素含量更高,但这是由裸盖菇素的酶促破坏引起的人为现象。用稀醋酸提取比用含醇的水效果更好。用甲醇进行定量提取的简单一步法仍然是从真菌生物质中获得真正生物碱的最安全方法。

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