已知最小的真核生物基因组编码一种蛋白质基因：迈向对核质体功能的理解。

The smallest known eukaryotic genomes encode a protein gene: towards an understanding of nucleomorph functions.

作者信息

Hofmann C J, Rensing S A, Häuber M M, Martin W F, Müller S B, Couch J, McFadden G I, Igloi G L, Maier U G

机构信息

Institut für Biologie II/Zellbiologie, Freiburg, Germany.

出版信息

Mol Gen Genet. 1994 Jun 3;243(5):600-4. doi: 10.1007/BF00284209.

DOI:10.1007/BF00284209

PMID:8208251

Abstract

Cryptomonads are unicellular algae with plastids surrounded by four membranes. Between the two pairs of membranes lies a periplastidal compartment that harbours a DNA-containing organelle, termed the nucleomorph. The nucleomorph is the vestigial nucleus of a phototrophic, eukaryotic endosymbiont. Subcloning of parts of one nucleomorph chromosome revealed a gene coding for an Hsp70 protein. We demonstrate the expression of this nucleomorph protein-coding gene and present a model for protein transport from the host to the endosymbiont compartment.

摘要

隐藻是单细胞藻类，其质体被四层膜包围。在两对膜之间有一个周质体间隔，其中含有一个含DNA的细胞器，称为核质体。核质体是光合营养真核内共生体的残余细胞核。对一条核质体染色体部分片段的亚克隆揭示了一个编码热休克蛋白70（Hsp70）的基因。我们证明了该核质体蛋白质编码基因的表达，并提出了一个蛋白质从宿主运输到内共生体间隔的模型。