阿尔茨海默病中血小板细胞色素c氧化酶活性降低。

Reduced platelet cytochrome c oxidase activity in Alzheimer's disease.

作者信息

Parker W D, Mahr N J, Filley C M, Parks J K, Hughes D, Young D A, Cullum C M

机构信息

Department of Neurology, University of Colorado School of Medicine, Denver.

出版信息

Neurology. 1994 Jun;44(6):1086-90. doi: 10.1212/wnl.44.6.1086.

DOI:10.1212/wnl.44.6.1086

PMID:8208406

Abstract

We evaluated a simplified method for preparation and analysis of platelet cytochrome c oxidase activity in Alzheimer's disease (AD) and control patients. Mean cytochrome c oxidase activity in controls (n = 17) was 0.233 sec-1/mg whereas mean cytochrome c oxidase activity in Alzheimer patients (n = 19) was 0.193 sec-1/mg, p = 0.033. Complex III (ubiquinol:cytochrome c oxidoreductase), complex II (succinic dehydrogenase), and citrate synthase were all assayed as internal controls and were not significantly different in controls and Alzheimer patients. There is a relatively specific loss of platelet cytochrome c oxidase activity in Alzheimer disease patients.

摘要

我们评估了一种用于制备和分析阿尔茨海默病（AD）患者及对照患者血小板细胞色素c氧化酶活性的简化方法。对照组（n = 17）的细胞色素c氧化酶平均活性为0.233秒⁻¹/毫克，而阿尔茨海默病患者（n = 19）的细胞色素c氧化酶平均活性为0.193秒⁻¹/毫克，p = 0.033。复合物III（泛醇：细胞色素c氧化还原酶）、复合物II（琥珀酸脱氢酶）和柠檬酸合酶均作为内部对照进行检测，在对照组和阿尔茨海默病患者中无显著差异。阿尔茨海默病患者血小板细胞色素c氧化酶活性存在相对特异性的降低。