仓鼠颊囊黏膜的长期器官培养。
Long-term organ culture of hamster cheek pouch mucosa.
作者信息
Mock D, Main J H
出版信息
J Oral Pathol. 1976 Jul;5(4):237-40. doi: 10.1111/j.1600-0714.1976.tb01770.x.
Abstract
Explants of cheek pouch mucosa from 12- to 48-hour-old hamsters have been maintained in organ culture for up to 49 days with excellent preservation of tissue integrity and relationships. The technique uses gelatin sponge matrix in Leighton tubes with Eagle's Minimum Essential Medium (M.E.M.) supplemented with 10% calf serum and antibiotics.
摘要
来自12至48小时龄仓鼠的颊囊黏膜外植体已在器官培养中维持长达49天，组织完整性和结构关系保存良好。该技术使用Leighton管中的明胶海绵基质，培养基为添加了10%小牛血清和抗生素的伊格尔氏最低必需培养基（M.E.M.）。
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