Grosse B, Siddell S G
Institut für Virologie, Universität Würzburg.
Adv Exp Med Biol. 1993;342:177-82. doi: 10.1007/978-1-4615-2996-5_28.
A monoclonal antibody resistant (MAR) variant of MHV was isolated after infection of hybridoma cells secreting the neutralizing and fusion-inhibiting monoclonal antibody, mAb 11F. The isolated variant was able to mediate syncytia formation even in the presence of high concentrations of mAb 11F. The S gene of the variant was cloned and sequenced. There were three nucleotide exchanges in comparison to the wild-type S gene, resulting in two amino acid alterations. However, both amino acid substitutions (at positions 255 and 1116) were located outside the binding site of mAb 11F.
在分泌中和及融合抑制单克隆抗体mAb 11F的杂交瘤细胞感染后，分离出了一株MHV的单克隆抗体抗性（MAR）变体。即使在高浓度mAb 11F存在的情况下，分离出的变体仍能介导合胞体形成。对该变体的S基因进行了克隆和测序。与野生型S基因相比，有三个核苷酸交换，导致两个氨基酸改变。然而，这两个氨基酸取代（位于第255和1116位）都位于mAb 11F的结合位点之外。
