源自中枢神经系统的JHM病毒特异性细胞毒性T细胞。

JHM virus-specific cytotoxic T cells derived from the central nervous system.

作者信息

Stohlman S, Bergmann C, LaMonica N, Lai M, Yeh J, Kyuwa S

机构信息

Department of Neurology, University of Southern California School of Medicine.

出版信息

Adv Exp Med Biol. 1993;342:419-23. doi: 10.1007/978-1-4615-2996-5_66.

DOI:10.1007/978-1-4615-2996-5_66

PMID:8209762

Abstract

Spleen cells cultured from Balb/c mice immunized with the JHM strain of mouse hepatitis virus (JHMV) have CD8+ cytotoxic T cells (CTL) specific for both the S and N proteins, but not the M or HE proteins. T cell lines were established from the brains of Balb/c mice infected with JHMV. The majority of the lines (20 of 22) were specific for JHMV. Analysis of the viral structural proteins which served as target structures indicate that most (15 of 20) were specific for the N protein. One line was specific for the S protein and four lines were specific for JHMV but the protein recognized could not be determined. These data suggest that early during infection there is a preferential recruitment of N protein specific CTL into the CNS of infected mice.

摘要

用小鼠肝炎病毒（JHMV）的JHM株免疫的Balb/c小鼠培养的脾细胞具有针对S和N蛋白的CD8 + 细胞毒性T细胞（CTL），但不针对M或HE蛋白。从感染JHMV的Balb/c小鼠的脑中建立T细胞系。大多数细胞系（22个中的20个）对JHMV具有特异性。作为靶结构的病毒结构蛋白分析表明，大多数（20个中的15个）对N蛋白具有特异性。一个细胞系对S蛋白具有特异性，四个细胞系对JHMV具有特异性，但无法确定所识别的蛋白。这些数据表明，在感染早期，N蛋白特异性CTL优先募集到受感染小鼠的中枢神经系统中。