Lloveras B, Garin-Chesa P, Myc A, Melamed M
Ciudad Sanitaria de Bellvitge, L'Hospitalet de Llobregat, Barcelona, Spain.
Am J Clin Pathol. 1994 Jun;101(6):703-7. doi: 10.1093/ajcp/101.6.703.
Proliferative fraction has been defined as an independent prognostic marker for some malignant neoplasms. An estimate of the proliferative fraction can be obtained by cell-cycle analysis of flow cytometric DNA measurements. However, overlapping DNA distributions of aneuploid neoplasms are difficult to analyze, even with sophisticated computer programs, and results are not always reproducible. Bromodeoxyuridine (BrdUrd) labeling followed by immunohistochemical detection has been proposed as an alternative, simple, and accurate method for identifying and counting DNA synthesizing cells. In vitro BrdUrd labeling was performed on 87 tumors, including 35 lung cancers, 25 breast carcinomas, and 27 tumors of other origin. Results were compared with flow cytometric S-phase estimates in 46 cases. Mean BrdUrd labeling of lung tumors was 8.5% +/- 5.2%, compared with a mean flow cytometric S-phase fraction of 20% +/- 18.4%. Mean BrdUrd labeling of breast carcinomas was 6.8% +/- 3.8%, compared with a mean flow cytometric S-phase fraction of 12.5% +/- 9.9%. In both tumor types, BrdUrd labeling correlated well with histologic grade. Correlation between BrdUrd labeling and flow cytometric S-phase estimates were generally poor, particularly with aneuploid tumors.
增殖分数已被定义为某些恶性肿瘤的独立预后标志物。通过对流式细胞术DNA测量结果进行细胞周期分析，可以获得增殖分数的估计值。然而，即使使用复杂的计算机程序，非整倍体肿瘤重叠的DNA分布也很难分析，而且结果并不总是可重复的。有人提出，采用溴脱氧尿苷（BrdUrd）标记后进行免疫组织化学检测，作为一种识别和计数DNA合成细胞的简单、准确的替代方法。对87例肿瘤进行了体外BrdUrd标记，其中包括35例肺癌、25例乳腺癌和27例其他来源的肿瘤。将46例病例的结果与流式细胞术S期估计值进行了比较。肺肿瘤的平均BrdUrd标记率为8.5%±5.2%，而流式细胞术S期平均分数为20%±18.4%。乳腺癌的平均BrdUrd标记率为6.8%±3.8%，而流式细胞术S期平均分数为12.5%±9.9%。在这两种肿瘤类型中，BrdUrd标记与组织学分级密切相关。BrdUrd标记与流式细胞术S期估计值之间的相关性通常较差，尤其是对于非整倍体肿瘤。
