Khan I, Hossain A, Whitman G F, Sarkar N H, McDonough P G
Department of Obstetrics and Gynecology, Medical College of Georgia, Augusta 30912.
Prostaglandins. 1993 Aug;46(2):139-44. doi: 10.1016/0090-6980(93)90039-a.
The influence of PGF2 alpha on c-jun gene expression in ovary, uterus and adrenal was examined. Three and seven day postovulatory PMSG primed immature rats received 500 ug PGF2 alpha by two subcutaneous injections 8 hours apart. Control rats received saline. Animals were sacrificed 30 minutes after the second injection of PGF2 alpha. Tissues were obtained and frozen in liquid nitrogen. RNA extracted from ovary, uterus and adrenal was analyzed by Northern and slot blot. c-jun was expressed differentially in these organs. An increase in c-jun expression by PGF2 alpha treatment occurred in the ovary but not in the adrenal and uterus. The effect of PGF2 alpha on c-jun was stronger in older compared to younger corpora lutea. These results indicate differential regulation of c-jun by PGF2 alpha in steroidogenic and steroid responsive tissues and that c-jun might be linked to the mode of action of PGF2 alpha in luteolysis.
研究了前列腺素F2α（PGF2α）对卵巢、子宫和肾上腺中c-jun基因表达的影响。对排卵后3天和7天的孕马血清促性腺激素（PMSG）预处理的未成熟大鼠，每隔8小时皮下注射500微克PGF2α，共注射两次。对照大鼠注射生理盐水。在第二次注射PGF2α后30分钟处死动物。获取组织并在液氮中冷冻。从卵巢、子宫和肾上腺提取的RNA通过Northern印迹和狭缝印迹进行分析。c-jun在这些器官中的表达存在差异。PGF2α处理使卵巢中c-jun表达增加，但在肾上腺和子宫中未增加。与年轻黄体相比，PGF2α对老年黄体中c-jun的作用更强。这些结果表明PGF2α在类固醇生成组织和类固醇反应性组织中对c-jun有不同的调节作用，并且c-jun可能与PGF2α在黄体溶解中的作用方式有关。
