Fernández V, Videla L A
Departamento de Bioquímica, Facultad de Medicina, Universidad de Chile, Santiago.
Toxicol Lett. 1993 Aug;69(2):205-10. doi: 10.1016/0378-4274(93)90105-7.
The effect of daily doses of 0.1 mg 3,3',5-triiodothyronine (T3)/kg for three consecutive days on hepatic O2 uptake was studied in the isolated perfused rat liver, in the absence and presence of the antioxidants desferrioxamine (DFO) and allopurinol (A). T3 treatment elicited a thermogenic condition in the animals, together with a 25% increase of total O2 consumption by the liver, which is inhibited by 0.5 mM DFO or 1 mM A. The antioxidant-sensitive respiration is enhanced by 62-64% by T3 over control values and represents 16-25% of the net increase in O2 uptake elicited by the hormone treatment. The respiratory components suppressed by the antioxidants are suggested to represent O2 equivalents related to T3-induced oxidative stress, and correlate with elevated rates of fractional lactate dehydrogenase efflux from the perfused livers.
在离体灌注大鼠肝脏中，研究了连续三天每天给予0.1 mg 3,3',5-三碘甲状腺原氨酸（T3）/kg对肝脏氧气摄取的影响，实验分别在不存在和存在抗氧化剂去铁胺（DFO）及别嘌呤醇（A）的情况下进行。T3处理使动物处于产热状态，同时肝脏的总氧气消耗量增加了25%，而0.5 mM DFO或1 mM A可抑制这种增加。与对照值相比，T3使对抗氧化剂敏感的呼吸增强了62% - 64%，且占激素处理引起的氧气摄取净增加量的16% - 25%。被抗氧化剂抑制的呼吸成分被认为代表与T3诱导的氧化应激相关的氧当量，并且与灌注肝脏中乳酸脱氢酶流出分数的升高速率相关。
