Aitouche A, Touraine J L
Immunobiology and Transplantation Unit, INSERM U80, CNRS URA-1177, UCBL, Hôpital Edouard Herriot, Lyon, France.
Transplantation. 1993 Sep;56(3):503-8. doi: 10.1097/00007890-199309000-00003.
Second-set rejection is generally considered to be mediated by cytotoxic humoral antibodies. A few discordant data have been reported, however. To address this question, we have taken advantage of a model in which specific cytotoxic alloantibodies are not produced although transplantation cellular immunity develops. Following a transplant of allogeneic stem cells from fetal liver, chimeric mice (BALB/c-->CBA--i.e., H-2d-->H-2k) were obtained; virtually all peripheral blood lymphocytes and spleen cells were of BALB/c donor origin. Anti-sheep red blood cell humoral response was present, although significantly lower in chimeras than in controls. These allochimeras were hyperimmunized by skin grafts and injections of spleen cells. The survival of skin grafts and the production of antibodies were then analyzed. When hyperimmunized against a third party, B6 (H-2b), chimeric mice were not able to raise a detectable humoral response involving anti-B6 cytotoxic antibodies, yet they rejected B6 skin allografts in an accelerated fashion (8.44 +/- 0.17 days). Control CBA mice rejecting second-set B6 skin grafts within the same delay developed high-titer, specific cytotoxic antisera (mean titer = 140). These data show that cytotoxic allospecific antibodies are not indispensable in the development of second-set accelerated rejection of skin allografts.
二次排斥反应通常被认为是由细胞毒性体液抗体介导的。然而，也有一些不一致的数据报道。为了解决这个问题，我们利用了一个模型，在该模型中，尽管移植细胞免疫发展，但不会产生特异性细胞毒性同种抗体。在移植来自胎肝的同种异体干细胞后，获得了嵌合小鼠（BALB/c→CBA，即H-2d→H-2k）；几乎所有外周血淋巴细胞和脾细胞都来自BALB/c供体。存在抗绵羊红细胞体液反应，尽管嵌合体中的反应明显低于对照组。这些异源嵌合体通过皮肤移植和脾细胞注射进行超免疫。然后分析皮肤移植的存活情况和抗体的产生。当针对第三方B6（H-2b）进行超免疫时，嵌合小鼠无法引发涉及抗B6细胞毒性抗体的可检测体液反应，但它们以加速方式排斥B6皮肤同种异体移植物（8.44±0.17天）。在相同延迟内排斥二次B6皮肤移植物的对照CBA小鼠产生了高滴度的特异性细胞毒性抗血清（平均滴度 = 140）。这些数据表明，细胞毒性同种特异性抗体在皮肤同种异体移植物二次加速排斥反应的发展中并非不可或缺。
