Reichenbach H D, Mödl J, Brem G
Department of Molecular Animal Breeding, Faculty of Veterinary Medicine, Ludwig-Maximilian University, München, Germany.
Vet Rec. 1993 Jul 10;133(2):36-9. doi: 10.1136/vr.133.2.36.
A method is described for the non-surgical transfer of embryos in pigs. Embryos at the 8-cell to the hatched blastocyst stage, recovered on days 4 to 7 of the oestrous cycle by flushing the oviducts and uteri of superovulated donors, were transferred transcervically into the uterine body of anaesthetised recipient gilts using a sterile disposable plastic spiral catheter and an embryo transfer cannula. Fifty-eight non-surgical transfers have been performed and six pregnancies were established. Eight and three normal fetuses were recovered from two recipients slaughtered between 35 and 45 days after embryo transfer. Three recipients came to term and gave birth to litters of two, six and seven living piglets. One recipient aborted between 45 and 60 days of gestation.
本文描述了一种猪胚胎非手术移植的方法。在发情周期的第4至7天，通过冲洗超排供体的输卵管和子宫，获取处于8细胞至孵化囊胚阶段的胚胎，使用无菌一次性塑料螺旋导管和胚胎移植套管经宫颈将胚胎移植到麻醉的受体后备母猪的子宫体中。已进行了58次非手术移植，成功建立了6例妊娠。在胚胎移植后35至45天之间屠宰的2头受体母猪中，分别回收了8头和3头正常胎儿。3头受体母猪足月分娩，分别产下2头、6头和7头存活仔猪。1头受体母猪在妊娠45至60天之间流产。
