Lajoie G, Zbieranowski I, Demianiuk C, Knape W A, Edinger M G, Tubbs R R, Murray D
Department of Pathology, St. Michael's Hospital, Toronto, Ontario, Canada.
Am J Clin Pathol. 1993 Oct;100(4):456-62. doi: 10.1093/ajcp/100.4.456.
The DNA ploidy status of 53 fresh primary breast carcinomas was analyzed in a comparative study of flow cytometric (FCM) and image cytometric (ICM) analyses. Samples for FCM analysis were obtained with an in vitro fine-needle aspiration technique. Touch imprints from the same tumors were analyzed by three independent observers by ICM analysis. An ICM comparative study of "sequential" and "visually selected" nuclei also was performed. There was an overall concordance of 0.85 in the classification of diploid and nondiploid tumors by FCM and ICM analyses. In most discordant cases, a nondiploid population was identified by FCM analysis alone. This is attributed to the superior resolution of and sampling/preparatory method used for FCM analysis. There was an overall concordance of 0.91 in the classification of diploid and nondiploid tumors by ICM analysis. Selective analysis of atypical nuclei resulted in increased sensitivity in the detection of DNA aneuploid populations by ICM analysis.
在一项流式细胞术（FCM）和图像细胞术（ICM）分析的对比研究中，对53例新鲜原发性乳腺癌的DNA倍体状态进行了分析。FCM分析的样本通过体外细针穿刺技术获取。来自相同肿瘤的触片由三名独立观察者进行ICM分析。还进行了“连续”和“视觉选择”细胞核的ICM对比研究。FCM和ICM分析在二倍体和非二倍体肿瘤分类中的总体一致性为0.85。在大多数不一致的病例中，仅通过FCM分析鉴定出非二倍体群体。这归因于FCM分析所使用的更高分辨率以及采样/制备方法。ICM分析在二倍体和非二倍体肿瘤分类中的总体一致性为0.91。对非典型细胞核的选择性分析导致ICM分析在检测DNA非整倍体群体时灵敏度增加。
