Properties of bispecific rosette-forming cells. III.---Antigen binding cells with double specificity after immunization with two covalently linked antigens.

Adult C57B1/6 mice were immunized with bovine serumalbumin and horse radish peroxidase administered simultaneously but either separated or covalently linked by glutaraldehyde treatment. The percentage of double rosettes formed with both antigens (coupled to red cells) as compared to the total rosette forming cells reached 20% when mice had been injected by the conjugate whereas it remained less than 2% in case of separate antigen administrations. In addition, these bispecific rosettes were inhibited after anti-theta serum plus complement treatment.