Halm D R, Kirk K L, Sathiakumar K C
Department of Physiology, Ohio State University, Columbus 43210.
Am J Physiol. 1993 Sep;265(3 Pt 1):G423-31. doi: 10.1152/ajpgi.1993.265.3.G423.
Crypts of Lieberkühn were isolated from rabbit distal colon and the halide-sensitive dye 6-methoxy-N-[3-sulfopropyl]quinolinium was used to monitor changes in cell Cl by fluorescence microscopy. Distal colon from rabbits actively secretes Cl and K when stimulated with prostaglandin (PG) E2 but secretes only K in response to epinephrine. The secretagogues PGE2 and epinephrine each produced transient decreases of the apparent cell Cl concentration in about one-half of the crypt cells. Permeability to Cl was assessed by brief substitutions with gluconate or Br in the bath. After stimulation of secretion by PGE2 or epinephrine, Cl efflux and Br influx were increased but only in the cells that exhibited the decrease in cell Cl at the onset of stimulation. Although Cl efflux during gluconate substitution was stimulated similarly with either PGE2 or epinephrine, epinephrine stimulation led to a lower apparent Cl concentration after 2 min of gluconate substitution. Together these results support the concept that a particular epithelial cell type in the crypts responds to secretagogues and that the Cl permeability pathways differ between the secretory states induced by PGE2 and epinephrine.
从兔远端结肠分离出利伯kühn隐窝，使用卤化物敏感染料6-甲氧基-N-[3-磺丙基]喹啉鎓通过荧光显微镜监测细胞氯离子的变化。兔远端结肠在受到前列腺素（PG）E2刺激时会主动分泌氯离子和钾离子，但对肾上腺素的反应仅分泌钾离子。促分泌剂PGE2和肾上腺素分别使约一半的隐窝细胞中表观细胞氯离子浓度出现短暂下降。通过在浴液中短暂替换为葡萄糖酸盐或溴来评估氯离子通透性。在PGE2或肾上腺素刺激分泌后，氯离子外流和溴内流增加，但仅在刺激开始时表现出细胞氯离子减少的细胞中增加。尽管在葡萄糖酸盐替换期间，PGE2或肾上腺素对氯离子外流的刺激相似，但在葡萄糖酸盐替换2分钟后，肾上腺素刺激导致的表观氯离子浓度较低。这些结果共同支持了这样的概念，即隐窝中的特定上皮细胞类型对促分泌剂有反应，并且在PGE2和肾上腺素诱导的分泌状态之间，氯离子通透性途径有所不同。
