Anderson F L, Kralios A C, Reid B, Hanson G R
Department of Internal Medicine (Cardiology), University of Utah School of Medicine, Salt Lake City.
Am J Physiol. 1993 Sep;265(3 Pt 2):H959-65. doi: 10.1152/ajpheart.1993.265.3.H959.
The effects of right cervical vagal and left sympathetic stimulation on release of immunoreactive vasoactive intestinal peptide (VIP) and neuropeptide Y (NPY) into cardiac venous and lymphatic effluent was tested in 11 anesthetized adult mongrel dogs. After stimulation of the right cervical vagus (1 ms, 20 Hz, 5 V) for 3 min, VIP output in lymphatic effluent was significantly increased at 1.90 +/- 0.56 pg/min compared with control of 0.90 +/- 0.42 pg/min. NPY output in lymphatic effluent and VIP and NPY release into coronary venous effluent, as measured by the arterial-coronary sinus concentration difference, were not changed. After stimulation of the ansae of the left sympathetic ganglion (1 ms, 10 Hz, 5 V) for 3 min, NPY output in lymphatic effluent was significantly increased at 4.72 +/- 1.58 pg/min compared with a control of 0.73 +/- 0.66 pg/min. VIP output in lymphatic effluent was not changed. VIP arterial-coronary sinus concentration difference decreased slightly but significantly, and NPY arterial-coronary sinus concentration difference decreased markedly after left sympathetic stimulation. In three additional dogs in which coronary sinus blood flow was measured, NPY overflow during left sympathetic stimulation increased from 28.2 +/- 23.5 to 129.6 +/- 212.7 pg/min. Thus VIP and NPY release from the canine heart can be evoked by right cervical vagal and left sympathetic stimulation, respectively. VIP and NPY may play a role as cardiac noncholinergic-nonadrenergic neurotransmitters.
在11只麻醉的成年杂种犬身上测试了右颈迷走神经和左交感神经刺激对免疫反应性血管活性肠肽（VIP）和神经肽Y（NPY）释放到心脏静脉和淋巴流出液中的影响。刺激右颈迷走神经（1毫秒，20赫兹，5伏）3分钟后，淋巴流出液中的VIP输出显著增加，为1.90±0.56皮克/分钟，而对照组为0.90±0.42皮克/分钟。淋巴流出液中的NPY输出以及通过动脉-冠状窦浓度差测量的VIP和NPY释放到冠状静脉流出液中的量均未改变。刺激左交感神经节袢（1毫秒，10赫兹，5伏）3分钟后，淋巴流出液中的NPY输出显著增加，为4.72±1.58皮克/分钟，而对照组为0.73±0.66皮克/分钟。淋巴流出液中的VIP输出未改变。左交感神经刺激后，VIP动脉-冠状窦浓度差略有但显著下降，NPY动脉-冠状窦浓度差显著下降。在另外三只测量冠状窦血流量的犬中，左交感神经刺激期间NPY溢出从28.2±23.5增加到129.6±212.7皮克/分钟。因此，右颈迷走神经和左交感神经刺激分别可诱发犬心脏释放VIP和NPY。VIP和NPY可能作为心脏非胆碱能-非肾上腺素能神经递质发挥作用。
