Scott J A, Brogdon W G, Collins F H
Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia.
Am J Trop Med Hyg. 1993 Oct;49(4):520-9. doi: 10.4269/ajtmh.1993.49.520.
A ribosomal DNA-polymerase chain reaction (PCR) method has been developed for species identification of individuals of the five most widespread members of the Anopheles gambiae complex, a group of morphologically indistinguishable sibling mosquito species that includes the major vectors of malaria in Africa. The method, which is based on species-specific nucleotide sequences in the ribosomal DNA intergenic spacers, may be used to identify both species and interspecies hybrids, regardless of life stage, using either extracted DNA or fragments of a specimen. Intact portions of a mosquito as small as an egg or the segment of one leg may be placed directly into the PCR mixture for amplification and analysis. The method uses a cocktail of five 20-base oligonucleotides to identify An. gambiae, An. arabiensis, An. quadriannnulatus, and either An. melas in western Africa or An. melas in eastern and southern Africa.
已开发出一种核糖体DNA聚合酶链反应（PCR）方法，用于鉴别冈比亚按蚊复合体五个分布最广的成员的个体。这是一组形态上难以区分的同域蚊种，其中包括非洲疟疾的主要传播媒介。该方法基于核糖体DNA基因间隔区中的物种特异性核苷酸序列，可用于识别物种和种间杂种，无论处于何种生命阶段，使用提取的DNA或标本片段均可。小至一枚卵或一条腿段的完整蚊子部分可直接放入PCR混合物中进行扩增和分析。该方法使用一组由五条20个碱基的寡核苷酸组成的混合物来鉴别冈比亚按蚊、阿拉伯按蚊、四斑按蚊，以及西非的梅拉斯按蚊或东非和南非的梅拉斯按蚊。
