Sasano Y, Mizoguchi I, Furusawa M, Aiba N, Ohtani E, Iwamatsu Y, Kagayama M
2nd Department of Oral Anatomy, School of Dentistry, Tohoku University, Sendai, Japan.
Anat Embryol (Berl). 1993 Jul;188(1):31-9. doi: 10.1007/BF00191449.
The rat tracheal cartilage was shown to calcify during development. The process of calcification was characterized in terms of distribution of alkaline phosphatase (ALP) activity and alterations to immunolocalization of types I and II collagens and glycosaminoglycans of proteoglycans during the development of the tracheal cartilage, in comparison with calcification of the epiphyseal growth plate cartilage. ALP activity was not identified in the tracheal cartilage in the course of calcification, which therefore differed from that in the growth plate. The tracheal cartilage matrix was not resorbed or invaded by type I collagen during calcification. This suggests that no osteogenesis is involved in calcification of the cartilage. Immunoreactivity for type II collagen became weaker in the central region of the tracheal cartilage during development. No net loss of proteoglycans was identified with Alcian blue staining after calcification of the tracheal cartilage. Immunoreactivity for chondroitin 4-sulphate increased in the calcified tracheal cartilage, while reactivity for chondroitin 6-sulphate was weaker in the calcified area than in the surrounding uncalcified region of the tracheal cartilage. The alteration of the extracellular matrices during development may be involved in the calcification of the rat tracheal cartilage.
研究表明，大鼠气管软骨在发育过程中会发生钙化。与骨骺生长板软骨的钙化相比，在气管软骨发育过程中，根据碱性磷酸酶（ALP）活性的分布以及I型和II型胶原蛋白和蛋白聚糖糖胺聚糖免疫定位的变化来表征钙化过程。在钙化过程中，气管软骨中未发现ALP活性，因此与生长板中的情况不同。钙化过程中，气管软骨基质未被I型胶原蛋白吸收或侵入。这表明软骨钙化不涉及成骨过程。在发育过程中，气管软骨中央区域的II型胶原蛋白免疫反应性变弱。气管软骨钙化后，阿尔新蓝染色未发现蛋白聚糖有净损失。硫酸软骨素4的免疫反应性在钙化的气管软骨中增加，而硫酸软骨素6在钙化区域的反应性比气管软骨周围未钙化区域弱。发育过程中细胞外基质的变化可能与大鼠气管软骨的钙化有关。
