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从白血病细胞中分离富含高尔基体的组分。

Isolation of a Golgi-apparatus-enriched fraction from leukaemic cells.

作者信息

Warley A, Cook G M

出版信息

Biochem J. 1976 May 15;156(2):245-51. doi: 10.1042/bj1560245.

DOI:10.1042/bj1560245
PMID:821478
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1163743/
Abstract
  1. A Golgi-apparatus-enriched fraction was isolated from acute leukaemic lymphoblasts of AKR mice by using an homogenate stabilized with 1 mM-glutaraldehyde. 2. The isolated fraction, which was shown morphologically to be enriched in dictyosomes, possessed between 44- and 76-fold increase in specific activity, compared with the tumour homogenate, of UDP-galactose-glycoprotein galactosyltransferase and between 3- and10.5-fold increase in relative specific activity of UDP-N-acetygalactosamine-polypeptide N-acetylgalactosaminyltransferase. 3. Plasma membranes isolated from the leukaemic lymphoblasts also possessed glycoprotein galactosyltransferase activity, though in contrast with Golgi-apparatus-enriched material had no detectable polypeptide N-acetygalactosaminyltransferase. 4. The difficulties associated with maintaining the morphological integrity of the Golgi apparatus in subcellular fractionation are discussed.
摘要
  1. 通过使用用1 mM戊二醛稳定的匀浆，从AKR小鼠的急性白血病淋巴母细胞中分离出富含高尔基体的组分。2. 分离出的组分在形态学上显示富含高尔基体，与肿瘤匀浆相比，UDP-半乳糖-糖蛋白半乳糖基转移酶的比活性增加了44至76倍，UDP-N-乙酰半乳糖胺-多肽N-乙酰半乳糖胺基转移酶的相对比活性增加了3至10.5倍。3. 从白血病淋巴母细胞中分离出的质膜也具有糖蛋白半乳糖基转移酶活性，尽管与富含高尔基体的物质相比，没有可检测到的多肽N-乙酰半乳糖胺基转移酶。4. 讨论了在亚细胞分级分离中维持高尔基体形态完整性相关的困难。
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1ff/1163743/032197ba4279/biochemj00533-0067-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1ff/1163743/032197ba4279/biochemj00533-0067-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1ff/1163743/032197ba4279/biochemj00533-0067-a.jpg

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本文引用的文献

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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
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GLUTARALDEHYDE STABILIZATION AS AN AID TO GOLGI APPARATUS ISOLATION.戊二醛固定作为高尔基体分离的辅助手段
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BIOSYNTHESIS OF GLYCOPROTEINS. IV. THE SUBCELLULAR SITES OF INCORPORATION OF GLUCOSAMINE-1-14-C INTO GLYCOPROTEIN RAT LIVER.糖蛋白的生物合成。IV. 葡糖胺-1-¹⁴C掺入大鼠肝脏糖蛋白的亚细胞定位
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The electrophoretic mobility of normal and leukaemic cells of mice.小鼠正常细胞与白血病细胞的电泳迁移率
Biochem J. 1968 Apr;107(4):549-57. doi: 10.1042/bj1070549.
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Lymphocytes: circulation altered by trypsin.淋巴细胞：胰蛋白酶改变其循环。
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