Ramboarina C, Scheftel J M, Monteil H
Laboratoire de bactériologie de la faculté de médecine, université Louis-Pasteur, Strasbourg, France.
Ann Biol Clin (Paris). 1993;51(2):91-100.
Sixty-two Aeromonas strains (39 of clinical and 23 of environmental origin) were identified. The suicide phenomenon and autoagglutination were studied. Identification is based on esculin hydrolysis; fermentation of arabinose salicin, sucrose and mannitol; gas production from glucose, indole and beta hemolysis; Voges-Proskauer and decarboxylation reactions; and finally resistance to cephalothin (30 micrograms) and colistin (4 micrograms/ml). Thirty-four per cent of A hydrophila, 33% A caviae, 28% A veronii subspecies sobria, 3% A jandaei, 2% A veronii subspecies veronii were accurately identified. Also, several new species were identified such as A trota, A enteropelogenes, A schubertii, A ichthiosmia, according to the more recently proposed taxa. This identification scheme could enhance our knowledge concerning virulence factors, pathogenicity and environmental distribution.
共鉴定出62株气单胞菌菌株(39株来自临床,23株来自环境)。对自杀现象和自凝现象进行了研究。鉴定基于七叶苷水解;阿拉伯糖水杨苷、蔗糖和甘露醇发酵;葡萄糖产气、吲哚和β溶血;Voges-Proskauer反应和脱羧反应;最后是对头孢噻吩(30微克)和黏菌素(4微克/毫升)的耐药性。准确鉴定出34%的嗜水气单胞菌、33%的豚鼠气单胞菌、28%的维罗纳气单胞菌温和亚种、3%的詹氏气单胞菌、2%的维罗纳气单胞菌维罗纳亚种。此外,根据最近提出的分类群,还鉴定出了几个新物种,如特氏气单胞菌、肠道产气气单胞菌、舒伯特气单胞菌、鱼腥味气单胞菌。该鉴定方案可以增强我们对毒力因子、致病性和环境分布的了解。