Lee B W, Bey R F, Baarsch M J, Morrison R B, Freese W
Department of Veterinary PathoBiology, School of Veterinary Medicine, University of Minnesota, St Paul 55108.
Am J Vet Res. 1993 Aug;54(8):1270-6.
An ELISA-based method to estimate hemagglutination-inhibition (HI) titer was developed. Subtype specificity was obtained by using purified H1 and H3 hemagglutinin antigens. Using the linear relation that exists between ELISA and HI methods, regression lines for H1N1- and H3N2-monospecific porcine antisera were constructed. Approximation of actual HI titer could be obtained from insertion of ELISA values into the appropriate regression line. The HI estimations were within 50% of the actual measured HI value 84% of the time. In young pigs that had suckled immune sows, use of this ELISA revealed estimated HI titer > 320 at 2 and 4 weeks of age. After a typical farm outbreak of influenza A/swine (H1N1), estimated HI titers remain high for 4 to 6 months. Sub-type-specific estimation of the distribution frequency of positive influenza A (H1N1 or H3N2) results for sera from swine in regional herds indicated that 31.3 and 7.4% of the swine tested were positive (HI > 41) for H1N1 and H3N2, respectively. From these observations, we conclude that in many circumstances, an ELISA-based HI estimation method could be used as a substitute for the HI test.
建立了一种基于酶联免疫吸附测定（ELISA）的方法来估计血凝抑制（HI）效价。通过使用纯化的H1和H3血凝素抗原获得亚型特异性。利用ELISA和HI方法之间存在的线性关系，构建了H1N1和H3N2单特异性猪抗血清的回归线。将ELISA值代入适当的回归线即可获得实际HI效价的近似值。HI估计值在84%的时间内与实际测量的HI值相差在50%以内。在吮食免疫母猪乳汁的幼猪中，使用这种ELISA法显示在2周龄和4周龄时估计的HI效价>320。在典型的甲型流感/猪（H1N1）农场疫情爆发后，估计的HI效价在4至6个月内保持较高水平。对区域猪群血清中甲型流感（H1N1或H3N2）阳性结果分布频率的亚型特异性估计表明，分别有31.3%和7.4%的受试猪H1N1和H3N2呈阳性（HI>41）。基于这些观察结果，我们得出结论，在许多情况下，基于ELISA的HI估计方法可替代HI试验。
