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溶组织内阿米巴长期多宿主培养过程中同工酶模式的变化。

Change of isoenzyme pattern during long-term polyxenic cultivation of Entamoeba histolytica.

作者信息

Vrchotová N, Ditrich O, Giboda M

机构信息

Institute of Parasitology, Academy of Sciences of Czech Republic, Ceské Budĕjovice.

出版信息

Ann Parasitol Hum Comp. 1993;68(2):67-9. doi: 10.1051/parasite/199368267.

Abstract

Isoenzymes of phosphoglucomutase and hexokinase were repeatedly evaluated using starch gel electrophoresis in polyxenic cultures of Entamoeba histolytica. In two out of 18 strains spontaneous changes of isoenzyme patterns were recorded. While originally they were categorized into virulent group of zymodemes, following isoenzyme analysis classified them as non-virulent. The relation between virulence and isoenzyme pattern is questionable.

摘要

利用淀粉凝胶电泳对溶组织内阿米巴多菌培养物中的磷酸葡萄糖变位酶和己糖激酶同工酶进行了反复评估。在18个菌株中的2个菌株中记录到同工酶模式的自发变化。虽然它们最初被归类为酶型的强毒株,但经过同工酶分析后被归类为无毒株。毒力与同工酶模式之间的关系值得怀疑。

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