Carlier M F, Pantaloni D
Biochimie. 1976;58(1-2):27-33. doi: 10.1016/s0300-9084(76)80353-7.
The enzymatic activity of cytoplasmic NADP linked isocitrate deshydrogenase is under control of divalent metal cations. Both forms of tribasic isocitrate, free and metal chelated are used as alternative substrates with different turnover and affinity. The esential role of the metal cation is to increase the affinity of the substrate for the enzyme 70 times.