Salgado J, Villalaín J, Gómez-Fernández J C
Departamento de Bioquímica y Biología Molecular (A), Universidad de Murcia, Spain.
Arch Biochem Biophys. 1993 Nov 1;306(2):368-76. doi: 10.1006/abbi.1993.1525.
The effect of the association of alpha-tocopherol (vitamin E) with single chain or very asymmetric phospholipids has been studied by 31P nuclear magnetic resonance, Fourier transform infrared spectroscopy, and light microscopy. Our results demonstrate that alpha-tocopherol stabilizes and forms bilayer structures in systems composed of either 1-palmitoyl-sn-glycero-3-phosphocholine, 1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine, 1-O-hexadecyl-sn-glycero-3-phosphocholine, or 1,2-dipalmitoyl-sn-glycero-3- phosphocholine/1-palmitoyl-sn-glycero-3-phosphocholine, whereas in the absence of alpha-tocopherol all these systems produce micellar structures. The free hydroxyl group of alpha-tocopherol is shown to be important in stabilizing the bilayer structure by comparing the effects produced by an analog of alpha-tocopherol, alpha-tocopheryl acetate, which has the hydroxyl group blocked by an acetyl group. It is suggested that the complementary shapes of alpha-tocopherol and the asymmetric phospholipids may be the reason for the stabilization of the bilayer structure.
已通过³¹P核磁共振、傅里叶变换红外光谱和光学显微镜研究了α-生育酚（维生素E）与单链或非常不对称的磷脂结合的效果。我们的结果表明，α-生育酚在由1-棕榈酰-sn-甘油-3-磷酸胆碱、1-O-十六烷基-2-乙酰基-sn-甘油-3-磷酸胆碱、1-O-十六烷基-sn-甘油-3-磷酸胆碱或1,2-二棕榈酰-sn-甘油-3-磷酸胆碱/1-棕榈酰-sn-甘油-3-磷酸胆碱组成的体系中稳定并形成双层结构，而在没有α-生育酚的情况下，所有这些体系都会产生胶束结构。通过比较α-生育酚类似物α-生育酚乙酸酯（其羟基被乙酰基封闭）产生的效果，表明α-生育酚的游离羟基在稳定双层结构中很重要。有人认为，α-生育酚和不对称磷脂的互补形状可能是双层结构稳定的原因。
