Shirakawa H
Dept. of Urology, Jikei University School of Medicine.
Gan To Kagaku Ryoho. 1993 Oct;20(13):1945-52.
Surgical tumor specimens obtained from twenty-five patients with renal cell carcinoma were subjected to the gel-supported culture developed by Hoffman for a clinical approach. Cell viability was determined by exposure to 3H-thymidine. The anti-cancer drugs examined in this set were 13 in total: ADM, AMR, BLM, MMC, VCR, VLB, etoposide, CDDP, 5-FU, MTX, IFN-alpha, IFN-gamma and TNF. The specimens were exposed to media containing 1x and 1/10x achievable human plasma peak concentrations of the agents. The overall susceptibility rate for growing tumors obtained from patients was 88% (22 of 25 patients). Susceptibility (less than 50% of control 3H-thymidine uptake) to ADM was observed to be highest (56%) in 12 of 22 patients, and to VLB and BLM (50%) in 11 patients each. On the other hand, that to MTX was observed in 1 patient at the lowest rate (5%), and that to the BRM group drugs was observed in 3 in IFN-alpha (14%), 1 in IFN-gamma (5%) and 2 in TNF (9%). However the number of effective drugs was not correlated to the grade, architecture and cell type. These results suggest that this assay system may be useful for a chemosensitivity test.
从25例肾细胞癌患者获取的手术肿瘤标本，采用霍夫曼开发的凝胶支持培养法进行临床研究。通过暴露于³H-胸腺嘧啶核苷来测定细胞活力。本研究共检测了13种抗癌药物：阿霉素（ADM）、氨甲蝶呤（AMR）、博来霉素（BLM）、丝裂霉素（MMC）、长春新碱（VCR）、长春碱（VLB）、依托泊苷、顺铂（CDDP）、5-氟尿嘧啶（5-FU）、甲氨蝶呤（MTX）、α干扰素（IFN-α）、γ干扰素（IFN-γ）和肿瘤坏死因子（TNF）。将标本暴露于含有这些药物可达到的人体血浆峰值浓度1倍和1/10倍的培养基中。患者肿瘤生长的总体敏感率为88%（25例患者中的22例）。在22例患者中的12例中，观察到对阿霉素的敏感性（³H-胸腺嘧啶核苷摄取低于对照的50%）最高（56%），对长春碱和博来霉素的敏感性（50%）分别在11例患者中观察到。另一方面，对甲氨蝶呤的敏感性在1例患者中观察到最低率（5%），对生物反应调节剂（BRM）组药物的敏感性在α干扰素中有3例（14%）、γ干扰素中有1例（5%）、肿瘤坏死因子中有2例（9%）观察到。然而，有效药物的数量与分级、结构和细胞类型无关。这些结果表明，该检测系统可能有助于进行化学敏感性试验。
