细胞质pH调节大鼠巨核细胞中ATP诱导的钙依赖性钾电流振荡。
Cytoplasmic pH regulates ATP-induced Ca(2+)-dependent K(+)-current oscillation in rat megakaryocytes.
作者信息
Uneyama C, Uneyama H, Takahashi M, Akaike N
机构信息
Division of Pathology, National Institute of Health Sciences, Tokyo, Japan.
出版信息
Biochem J. 1993 Oct 1;295 ( Pt 1)(Pt 1):317-20. doi: 10.1042/bj2950317.
The effect of cytoplasmic pH (pH(i)) on ATP-induced Ca2+ oscillation of rat megakaryocytes was investigated by the whole-cell patch-clamp technique. Megakaryocytes responded to extracellular ATP and showed the periodic activation of K+ channels, which reflects the oscillation in intracellular free Ca2+ concentration. Intracellular alkalinization by 20 mM NH4Cl resulted in inhibition of the oscillatory response, and intracellular acidification by 20 mM sodium acetate enhanced the response. NH4Cl also inhibited the Ins(1,4,5)P3-induced oscillation. Sodium acetate had no effect on the InsP3-induced oscillation, but enhanced the guanosine 5'-[gamma-thio]triphosphate-induced response. These results indicate that pH(i) modulates the ATP-induced cellular response at least at two points.
采用全细胞膜片钳技术研究了细胞质pH值（pH(i)）对大鼠巨核细胞ATP诱导的Ca2+振荡的影响。巨核细胞对细胞外ATP有反应，并表现出K+通道的周期性激活，这反映了细胞内游离Ca2+浓度的振荡。20 mM NH4Cl引起的细胞内碱化导致振荡反应受到抑制，而20 mM醋酸钠引起的细胞内酸化则增强了反应。NH4Cl还抑制了Ins(1,4,5)P3诱导的振荡。醋酸钠对InsP3诱导的振荡没有影响，但增强了鸟苷5'-[γ-硫代]三磷酸诱导的反应。这些结果表明，pH(i)至少在两个点上调节ATP诱导的细胞反应。
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