Kwiatkowski L D, Noble R W
Veterans Administration Medical Center, Department of Medicine, Buffalo, New York.
Biochem Biophys Res Commun. 1993 Sep 30;195(3):1218-23. doi: 10.1006/bbrc.1993.2174.
At pH 6 in the presence of inositol hexaphosphate, IHP, conditions where ligand-saturated carp hemoglobin is already in the low affinity T state, the addition of glycerol has little effect on affinity and ligand binding remains noncooperative. At all other pH values examined, with and without IHP, the effect of glycerol is to lower oxygen affinity possibly by shifting the equilibrium between the T state and the high affinity R state in the direction of the T state. Although glycerol does not appear to have an appreciable effect on the T state itself, a small effect on the R state cannot be excluded by our data.
在pH值为6且存在肌醇六磷酸（IHP）的情况下，即配体饱和的鲤鱼血红蛋白已处于低亲和力T态的条件下，添加甘油对亲和力几乎没有影响，配体结合仍不具有协同性。在所有其他检测的pH值下，无论有无IHP，甘油的作用都是降低氧亲和力，可能是通过将T态和高亲和力R态之间的平衡向T态方向移动来实现的。尽管甘油似乎对T态本身没有明显影响，但我们的数据不能排除其对R态有微小影响的可能性。
