Izquierdo J M, Cuezva J M
Departamento de Biología Molecular, Universidad Autónoma de Madrid, Spain.
Biochem Biophys Res Commun. 1993 Oct 15;196(1):55-60. doi: 10.1006/bbrc.1993.2215.
We have previously shown that the nuclear-encoded gene's expression of mitochondrial beta-subunit of the F1-ATPase complex in rat liver is regulated at the translational level (Luis, A.M., Izquierdo, J.M., Ostronoff, L.K., Santarén, J., Salinas, M., and Cuezva, J.M. (1993) J. Biol. Chem. 268, 1868-1875). In this paper we report that the different steady-state levels of ATP synthase beta subunit mRNA detected in rat tissues are not paralleled by a proportional content of immunodetectable beta-F1-ATPase protein. The results suggest that tissue-specific transcriptional and post-transcriptional mechanisms contribute to differential mitochondrial biogenesis in mammalian cells. On the other hand, steady-state mRNA levels of the mitochondrial encoded ATP synthase subunits (ATP 6+8) indicate that nuclear and mitochondrial-encoded transcripts for this complex are in close relation, that is, the expression of both nuclear and mitochondrial genes is coordinated in all tissues examined.
我们之前已经表明，大鼠肝脏中F1 - ATP酶复合体线粒体β亚基的核编码基因的表达在翻译水平受到调控（路易斯，A.M.，伊兹基尔多，J.M.，奥斯特罗诺夫，L.K.，桑塔伦，J.，萨利纳斯，M.，以及奎兹瓦，J.M.（1993年）《生物化学杂志》268卷，第1868 - 1875页）。在本文中我们报道，在大鼠组织中检测到的ATP合酶β亚基mRNA的不同稳态水平，与免疫可检测的β - F1 - ATP酶蛋白的比例含量并不平行。结果表明，组织特异性转录和转录后机制有助于哺乳动物细胞中不同的线粒体生物发生。另一方面，线粒体编码的ATP合酶亚基（ATP 6 + 8）的稳态mRNA水平表明，该复合体的核编码和线粒体编码转录本密切相关，也就是说，在所有检测的组织中，核基因和线粒体基因的表达是协调的。
