Tomisawa H, Hayashi M, Fukushima M, Iida S, Uda F, Hattori K, Tateishi M
Upjohn Pharmaceuticals Limited Tsukuba Research Laboratories, Ibaraki, Japan.
Biochem Pharmacol. 1993 Oct 5;46(7):1113-7. doi: 10.1016/0006-2952(93)90457-8.
p-Bromothiophenol and S-(p-bromophenyl)-L-cysteine were formed enzymatically from S-(p-bromophenyl)-L-cysteine sulphoxide in the in vitro systems with isolated rat hepatocytes or purified cysteine conjugate beta-lyases. Isotope dilution study with non-radiolabelled carrier of each product suggested the initial liberation of the thiol and subsequent formation of the cysteine conjugate. C-S bond cleavage pathway to liberate sulphenic acid and thiol are postulated to play an important role in in vivo generation of toxic intermediates and products from cysteine conjugates.
在含有分离的大鼠肝细胞或纯化的半胱氨酸共轭β-裂解酶的体外系统中，对溴苯硫酚和S-（对溴苯基）-L-半胱氨酸由S-（对溴苯基）-L-半胱氨酸亚砜酶促形成。使用每种产物的非放射性标记载体进行的同位素稀释研究表明，首先释放出硫醇，随后形成半胱氨酸共轭物。推测释放亚磺酸和硫醇的C-S键裂解途径在体内由半胱氨酸共轭物生成有毒中间体和产物的过程中起重要作用。
