[Isolation of 6 subunits of scorpion, Androctonus australis garzonii, hemocyanin].

A method for the purification of the 5 sub-units 5 S and of a 7 S sub-unit of the haemocyanin of the Scorpion Androctonus australis garzonii is reported. It includes preparation of crude haemocyanin, dissociation in 1 M urea in the absence of divalent cations, starch gel electrophoresis and A 50 DEAE "Sephadex" separation. All the sub-units are homogeneous with regard to ultracentrifugation and polyacrylamide gel electrophoresis. The yield variers from 7 to 20 per cent according to each fraction.