Yilmaz A, Gaide A C, Sordat B, Borbenyi Z, Lahm H, Imam A, Schreyer M, Odartchenko N
Swiss Institute for Experimental Cancer Research, Epalinges.
Br J Cancer. 1993 Nov;68(5):868-73. doi: 10.1038/bjc.1993.447.
A human breast epithelial cell line (Hu-MI), established by microinjecting SV40 DNA into human milk epithelial cells, exhibits the phenotype of luminal epithelial cells and is neither clonogenic nor tumorigenic. From this cell line we have selected two sublines, HuMI-T and HuMI-TTul, reflecting different stages of spontaneous transformation. HuMI-T cells grow anchorage-independently, but do not induce tumours in nude mice. HuMI-TTul cells are clonogenic as well as tumorigenic. Cells from both lines exhibit polymorphic structural and numerical chromosome aberrations. Immortalisation of normal luminal epithelial cells from human mammary gland with SV40 DNA alone may thus cause random genetic changes eventually resulting in tumorigenic cell lines. Since Hu-MI, HuMI-T and HuMI-TTul represent some of the consecutive stages taking place during cellular transformation, they are particularly suited as a novel in vitro model system to study progression of human breast cancer.
通过将SV40 DNA显微注射到人乳腺上皮细胞中建立的人乳腺上皮细胞系（Hu-MI），表现出腔上皮细胞的表型，既无克隆形成能力也无致瘤性。从该细胞系中我们筛选出了两个亚系，HuMI-T和HuMI-TTul，它们反映了自发转化的不同阶段。HuMI-T细胞能够不依赖贴壁生长，但在裸鼠中不诱导肿瘤形成。HuMI-TTul细胞具有克隆形成能力且具有致瘤性。两个细胞系的细胞均表现出多态性的结构和数量染色体畸变。因此，仅用SV40 DNA使来自人乳腺的正常腔上皮细胞永生化可能会导致随机的基因变化，最终产生致瘤细胞系。由于Hu-MI、HuMI-T和HuMI-TTul代表了细胞转化过程中发生的一些连续阶段，它们特别适合作为一种新型的体外模型系统来研究人类乳腺癌的进展。
