• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

化学优化的抗肌球蛋白Fab与螯合聚合物的结合物：蛋白质-聚合物单点共价键的性质对生物分布和梗死定位的重要性。

Chemically optimized antimyosin Fab conjugates with chelating polymers: importance of the nature of the protein-polymer single site covalent bond for biodistribution and infarction localization.

作者信息

Trubetskoy V S, Narula J, Khaw B A, Torchilin V P

机构信息

Center for Imaging and Pharmaceutical Research, Massachusetts General Hospital, Charlestown 02129.

出版信息

Bioconjug Chem. 1993 Jul-Aug;4(4):251-5. doi: 10.1021/bc00022a001.

DOI:10.1021/bc00022a001
PMID:8218480
Abstract

Murine antimyosin Fab fragment was conjugated with 111In-labeled N-terminal-modified DTPA-polylysine using three bifunctional reagents: N-hydroxysuccinimide esters of 3-(2-pyridyldithio)propionic acid (SPDP conjugate), 4-(maleimidomethyl)cyclohexanecarboxylic acid (SMCC conjugate) and bromoacetic acid (BrAc conjugate) for potential localization of experimental myocardial infarction. Using various antibody preparations and a rabbit acute myocardial infarction model the following parameters were observed: (1) an in vitro antigen binding activity of SPDP conjugate = SMCC conjugate > BrAc conjugate, (2) a blood clearance rate of SPDP conjugate > BrAc conjugate > SMCC conjugate, (3) a liver and splenic accumulation of SPDP conjugate > BrAc conjugate > SMCC conjugate, and (4) the infarcted tissue activity showed an accumulation of SMCC conjugate > SPDP conjugate > BrAc conjugate. This study exemplifies the importance of rational chemical design of antimyosin Fab-chelating polymer conjugate for improved target tissue localization in vivo.

摘要

使用三种双功能试剂，将小鼠抗肌球蛋白Fab片段与111In标记的N端修饰的二乙三胺五乙酸-聚赖氨酸结合，用于实验性心肌梗死的潜在定位。这三种双功能试剂分别是：3-(2-吡啶二硫代)丙酸N-羟基琥珀酰亚胺酯（SPDP结合物）、4-(马来酰亚胺甲基)环己烷羧酸（SMCC结合物）和溴乙酸（BrAc结合物）。利用各种抗体制剂和兔急性心肌梗死模型，观察了以下参数：(1) SPDP结合物 = SMCC结合物 > BrAc结合物的体外抗原结合活性；(2) SPDP结合物 > BrAc结合物 > SMCC结合物的血液清除率；(3) SPDP结合物 > BrAc结合物 > SMCC结合物在肝脏和脾脏的蓄积；(4) 梗死组织活性显示SMCC结合物 > SPDP结合物 > BrAc结合物的蓄积。本研究例证了抗肌球蛋白Fab-螯合聚合物结合物的合理化学设计对于改善体内靶组织定位的重要性。

相似文献

1
Chemically optimized antimyosin Fab conjugates with chelating polymers: importance of the nature of the protein-polymer single site covalent bond for biodistribution and infarction localization.化学优化的抗肌球蛋白Fab与螯合聚合物的结合物：蛋白质-聚合物单点共价键的性质对生物分布和梗死定位的重要性。
Bioconjug Chem. 1993 Jul-Aug;4(4):251-5. doi: 10.1021/bc00022a001.
2
In vivo targeting of acute myocardial infarction with negative-charge, polymer-modified antimyosin antibody: use of different cross-linkers.带负电荷的聚合物修饰抗肌球蛋白抗体对急性心肌梗死的体内靶向作用：不同交联剂的应用
J Nucl Cardiol. 1995 Jan-Feb;2(1):26-34. doi: 10.1016/s1071-3581(05)80005-4.
3
Use of isothiocyanatobenzyl-DTPA derivatized monoclonal antimyosin fab for enhanced in vivo target localization.异硫氰酸苄基 - DTPA 衍生化单克隆抗肌球蛋白 Fab 片段用于增强体内靶点定位
J Nucl Med. 1990 Feb;31(2):211-7.
4
Modification of monoclonal antimyosin antibody: enhanced specificity of localization and scintigraphic visualization in acute experimental myocardial infarction.单克隆抗肌球蛋白抗体的修饰：急性实验性心肌梗死中定位特异性及闪烁显像的增强
J Mol Cell Cardiol. 1989 Feb;21 Suppl 1:31-5. doi: 10.1016/0022-2828(89)90835-3.
5
Terminal-modified polylysine-based chelating polymers: highly efficient coupling to antibody with minimal loss in immunoreactivity.末端修饰的基于聚赖氨酸的螯合聚合物：与抗体高效偶联，免疫反应性损失最小。
Bioconjug Chem. 1991 Sep-Oct;2(5):342-8. doi: 10.1021/bc00011a009.
6
Gamma imaging with negatively charge-modified monoclonal antibody: modification with synthetic polymers.
J Nucl Med. 1991 Sep;32(9):1742-51.
7
Rapid infarct imaging with a technetium-99m-labeled antimyosin recombinant single-chain Fv: evaluation in a canine model of acute myocardial infarction.用锝-99m标记的抗肌凝蛋白重组单链Fv进行快速梗死成像：在急性心肌梗死犬模型中的评估
J Nucl Med. 1993 Feb;34(2):234-41.
8
Imaging of cardiac transplantation rejection in primates using two new antimyosin agents.使用两种新型抗肌凝蛋白制剂对灵长类动物心脏移植排斥反应进行成像
J Nucl Med. 1992 Nov;33(11):1994-9.
9
Site-specific conjugation of chain-terminal chelating polymers to Fab' fragments of anti-CEA mAb: effect of linkage type and polymer size on conjugate biodistribution in nude mice bearing human colorectal carcinoma.链末端螯合聚合物与抗癌胚抗原单克隆抗体Fab'片段的位点特异性偶联：连接类型和聚合物大小对荷人结肠癌裸鼠体内偶联物生物分布的影响
Bioconjug Chem. 1992 Nov-Dec;3(6):477-83. doi: 10.1021/bc00018a003.
10
Localization of cardiac myosin-specific antibody in myocardial infarction.心肌肌球蛋白特异性抗体在心肌梗死中的定位
J Clin Invest. 1976 Aug;58(2):439-46. doi: 10.1172/JCI108488.

引用本文的文献

1
Bispecific antibody complex pre-targeted delivery of polymer-drug conjugates for cancer therapy.双特异性抗体复合物前靶向递聚合物药物偶联物用于癌症治疗。
Drug Deliv Transl Res. 2012 Feb;2(1):65-76. doi: 10.1007/s13346-011-0055-x.
2
Vascular targeting of nanocarriers: perplexing aspects of the seemingly straightforward paradigm.纳米载体的血管靶向：看似简单的范例中令人困惑的方面。
ACS Nano. 2014 May 27;8(5):4100-32. doi: 10.1021/nn500136z. Epub 2014 May 7.
3
In vivo targeting of acute myocardial infarction with negative-charge, polymer-modified antimyosin antibody: use of different cross-linkers.
带负电荷的聚合物修饰抗肌球蛋白抗体对急性心肌梗死的体内靶向作用：不同交联剂的应用
J Nucl Cardiol. 1995 Jan-Feb;2(1):26-34. doi: 10.1016/s1071-3581(05)80005-4.