Ugarova N N, Brovko L Iu, Kutuzova G D
Biokhimiia. 1993 Aug;58(9):1351-72.
Recent data from gene engineering, kinetic and fluorescent studies concerning the structure and functions of firefly luciferase are reviewed. Some new trends in the development of bioluminescent methods of control over bacterial contaminations, dynamics of intracellular processes and methods of bioluminescent detection in immuno- and DNA-assays are described. Possible applications of luciferase genes as markers are considered.
综述了有关萤火虫荧光素酶结构和功能的基因工程、动力学及荧光研究的最新数据。描述了在控制细菌污染的生物发光方法、细胞内过程动力学以及免疫分析和DNA分析中的生物发光检测方法等方面的一些新趋势。还考虑了荧光素酶基因作为标记物的可能应用。