Maguire K, Tuckwell V, Pereira A, Dean B, Singh B
Department of Psychiatry, University of Melbourne, Victoria, Australia.
Biol Psychiatry. 1993 Sep 15;34(6):356-60. doi: 10.1016/0006-3223(93)90179-h.
The lack of correlation between 5-HT uptake by platelets and the ability of platelet membrane to bind antidepressant drugs, particularly imipramine, has been reported. However, more recently it has been suggested that 3H-paroxetine could be a better drug with which to study the platelet 5-HT uptake mechanism in disease states. We have therefore compared the ability of platelet-rich plasma (PRP) from normal individuals to take up 5-HT with the ability of platelet membranes to bind paroxetine. A significant correlation was apparent between the Vmax of 14C-5-HT uptake by PRP and the Bmax of 3H-paroxetine binding to platelet membrane from 30 individuals (r = 0.6468, p = 0.0001). Furthermore, this correlation was highly significant in the 20 female (r = 0.7768, p = 0.00006) but not in the 10 male volunteers. There was also a significant association between Vmax and Bmax and the month of blood sampling but this did not totally account for the correlation between Vmax and Bmax. The simultaneous measurement of 5-HT uptake by PRP and paroxetine binding to platelet membranes from both depressed patients and matched controls should be carried out to confirm and extend these findings.
已有报道称血小板对5-羟色胺（5-HT）的摄取与血小板膜结合抗抑郁药物（尤其是丙咪嗪）的能力之间缺乏相关性。然而，最近有人提出，3H-帕罗西汀可能是一种更好的药物，可用于研究疾病状态下血小板5-HT的摄取机制。因此，我们比较了正常个体富含血小板血浆（PRP）摄取5-HT的能力与血小板膜结合帕罗西汀的能力。30名个体的PRP对14C-5-HT的摄取Vmax与3H-帕罗西汀与血小板膜的结合Bmax之间存在显著相关性（r = 0.6468，p = 0.0001）。此外，这种相关性在20名女性中高度显著（r = 0.7768，p = 0.00006），但在10名男性志愿者中不显著。Vmax和Bmax与采血月份之间也存在显著关联，但这并不能完全解释Vmax和Bmax之间的相关性。应该对抑郁症患者和匹配的对照组同时进行PRP摄取5-HT和血小板膜结合帕罗西汀的测量，以证实和扩展这些发现。
