Ultrastructural filament organization of hepatocyte cytoplasm revealed by embedment-free sections of rat liver.

The cytoplasmic matrix of rat liver cells was examined by embedment-free electron microscopy of polyethylene glycol (PEG) embedded tissue from which the PEG had been removed subsequently. Non-ionic detergent extraction of fresh blocks of tissue permitted the major cytoskeletal components to be visualized in relatively thick embedment-free sections. Immunolabelling confirmed the presence of the major cytoskeletal proteins (actin, cytokeratin and tubulin) in these sections. Hence, this method permits the examination of the ultrastructural organization of the cytoskeleton of the hepatocyte in blocks of fresh hepatic tissue.