Seeds E A, Hanss J, Page C P
Department of Pharmacology, King's College, University of London, UK.
J Lipid Mediat. 1993 Jul;7(3):269-78.
Exposure of normal guinea pigs to an aerosol of PAF induced a selective increase in the percentage of eosinophils in bronchoalveolar lavage (BAL) fluid 24 h after challenge. Challenge of actively sensitised guinea pigs with an aerosol of ovalbumin also induced a selective increase in the percentage of eosinophils recovered in BAL fluid 24 h post challenge. Pretreatment of actively sensitised guinea pigs or normal guinea pigs with unfractionated heparin significantly reduced such eosinophil infiltration induced by allergen or PAF challenge respectively, although higher amounts of heparin were required to inhibit antigen induced eosinophil infiltration. Similar effects were also observed following treatment with the low molecular weight heparin-like material ORG 10172 but not the anionic molecule polyglutamic acid or high molecular weight dextrans. These results suggest that proteoglycans may possess anti-allergic activity that is not necessarily related to either such molecules being anionic in nature nor to anti-coagulant activity.
将正常豚鼠暴露于PAF气雾剂中，在激发后24小时，支气管肺泡灌洗（BAL）液中嗜酸性粒细胞的百分比出现选择性增加。用卵清蛋白气雾剂激发主动致敏的豚鼠，在激发后24小时，回收的BAL液中嗜酸性粒细胞的百分比也出现选择性增加。用未分级肝素对主动致敏的豚鼠或正常豚鼠进行预处理，可分别显著减少由变应原或PAF激发诱导的嗜酸性粒细胞浸润，尽管抑制抗原诱导的嗜酸性粒细胞浸润需要更高剂量的肝素。用低分子量类肝素物质ORG 10172处理后也观察到类似效果，但阴离子分子聚谷氨酸或高分子量右旋糖酐则无此效果。这些结果表明，蛋白聚糖可能具有抗过敏活性，这不一定与这些分子本身呈阴离子性质或与抗凝活性有关。
