人血浆中草花粉特异性免疫球蛋白的同型分析。2. IgE、IgM、IgA类及IgG亚类抗体的定量分析。

Isotypic analysis of grass-pollen-specific immunoglobulins in human plasma. 2. Quantification of the IgE, IgM, IgA class and the IgG subclass antibodies.

作者信息

Batard T, Basuyaux B, Laroze A, Lambin P, Brémard-Oury C, Aucouturier P, Hamilton R G, David B, Peltre G

机构信息

Unité d'Immuno-Allergie, Institut Pasteur, Paris, France.

出版信息

Int Arch Allergy Immunol. 1993;102(3):279-87. doi: 10.1159/000236537.

DOI:10.1159/000236537

PMID:8219781

Abstract

Previously, the specificity of human immune responses to Dactylis pollen was analyzed in 26 plasma samples with high levels of grass-pollen-specific IgG4 ('IgG4+ plasma', largely from grass-pollen-allergic patients), as compared to 25 plasma samples with low grass-pollen-specific IgG4 ('normal plasma', from nonatopic individuals). In the present study, a quantification of the Dactylis-pollen-specific IgE, IgM, IgA class and IgG subclass antibodies in these plasma samples is proposed. Isotypic distribution in IgG4+ plasma was 68% IgG [IgG2 (38%) > IgG4 (30%) > IgG1 (19%) > IgG3 (13%)], 27% IgM, 4% IgA and 0.05% IgE. In normal plasma it was 73% IgM, 20% IgG [IgG3 (38%) > IgG2 (33%) > IgG1 (29%) > IgG4 (0%)], 6% IgA and 0.006% IgE. In IgG4+ plasma, specific IgE, IgG1, IgG2 and IgG4 concentrations were positively correlated between each other. Finally, the present study clearly confirmed the possible role of the CH gene regulation in allergic diseases.

摘要

此前，对26份草花粉特异性IgG4水平较高的血浆样本（“IgG4+血浆”，主要来自草花粉过敏患者）和25份草花粉特异性IgG4水平较低的血浆样本（“正常血浆”，来自非特应性个体）中人类对鸭茅花粉免疫反应的特异性进行了分析。在本研究中，提议对这些血浆样本中的鸭茅花粉特异性IgE、IgM、IgA类和IgG亚类抗体进行定量分析。IgG4+血浆中的同型分布为68% IgG [IgG2（38%）> IgG4（30%）> IgG1（19%）> IgG3（13%）]、27% IgM、4% IgA和0.05% IgE。在正常血浆中，其分布为73% IgM、20% IgG [IgG3（38%）> IgG2（33%）> IgG1（29%）> IgG4（0%）]、6% IgA和0.006% IgE。在IgG4+血浆中，特异性IgE、IgG1、IgG2和IgG4浓度之间呈正相关。最后，本研究明确证实了CH基因调控在过敏性疾病中的可能作用。