Finnegan M C, Goepel J R, Hancock B W, Goyns M H
Department of Clinical Oncology, Royal Hallamshire Hospital, Sheffield, England, UK.
Leuk Lymphoma. 1993 Jul;10(4-5):387-93. doi: 10.3109/10428199309148565.
Studies of quantitative changes in gene expression in malignant cells have often used housekeeping genes as controls against which the level of expression of a gene under study could be compared. We have now examined whether the expression of the most commonly used of these housekeeping genes can be regarded as reliable controls for gene expression studies in non-Hodgkin's lymphoma (NHL). We have used Northern blot analysis to compare the levels of expression of beta-actin, alpha-tubulin, beta 2-microglobulin and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) to that of ribosomal RNA. These studies demonstrated that whereas there was a reasonable correlation between the relative levels of rRNA and housekeeping gene expression in reactive hyperplastic nodes, there were major differences in the relative levels of expression of the housekeeping genes in both low and high grade lymphomas; only GAPDH showed any degree of consistency. These observations indicated that housekeeping gene expression was not a reliable control for estimating changes in the level of expression of other genes in NHL, and instead suggested that 18S or 28S rRNA expression offered a more accurate method of RNA quantitation.
对恶性细胞中基因表达定量变化的研究常常将管家基因用作对照，据此可比较所研究基因的表达水平。我们现在研究了这些常用管家基因的表达是否可被视为非霍奇金淋巴瘤（NHL）基因表达研究的可靠对照。我们使用Northern印迹分析比较了β-肌动蛋白、α-微管蛋白、β2-微球蛋白和甘油醛-3-磷酸脱氢酶（GAPDH）与核糖体RNA的表达水平。这些研究表明，虽然在反应性增生性淋巴结中rRNA和管家基因表达的相对水平之间存在合理的相关性，但在低度和高度淋巴瘤中管家基因的相对表达水平存在重大差异；只有GAPDH表现出一定程度的一致性。这些观察结果表明，管家基因表达并非估算NHL中其他基因表达水平变化的可靠对照，相反，提示18S或28S rRNA表达提供了一种更准确的RNA定量方法。
