一种准确扩增多态性CA重复序列的方法。
A method for accurate amplification of polymorphic CA-repeat sequences.
作者信息
Odelberg S J, White R
机构信息
Department of Human Genetics, University of Utah, Salt Lake City 84112.
出版信息
PCR Methods Appl. 1993 Aug;3(1):7-12. doi: 10.1101/gr.3.1.7.
Anomalous PCR products are often produced during the amplification of d(CA)n.d(TG)n sequences. Upon denaturing polyacrylamide gel electrophoresis, these products yield a ladder-like pattern that can complicate genotypic interpretation. We have developed two related techniques, referred to as two- and three-stage linear amplification (2-SLA and 3-SLA, respectively), which largely overcome this problem and yield readily interpretable banding patterns.
在d(CA)n.d(TG)n序列的扩增过程中常常会产生异常的PCR产物。在变性聚丙烯酰胺凝胶电泳时，这些产物会产生一种梯状模式，这可能会使基因型解读变得复杂。我们开发了两种相关技术，分别称为两阶段线性扩增和三阶段线性扩增（分别为2-SLA和3-SLA），它们在很大程度上克服了这个问题，并产生易于解读的条带模式。
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