流式细胞术在细胞功能测量中的贡献。

Flow cytometry's contribution to the measurement of cell functions.

作者信息

Maftah A, Huet O, Gallet P F, Ratinaud M H

机构信息

Institut de Biotechnologie, Limoges, France.

出版信息

Biol Cell. 1993;78(1-2):85-93. doi: 10.1016/0248-4900(93)90119-y.

DOI:10.1016/0248-4900(93)90119-y

PMID:8220230

Abstract

Flow cytometry has important advantages over conventional techniques. It is rapid, highly sensitive and allows multi-parametric analysis and cell sorting. Potential exists for the measurement of many cell functions by flow cytometry. The technique can be used to determine cell viability, intracellular calcium and pH, membrane potential, enzyme activities, membrane fluidity and endocytosis. Numerous examples are given on the applications of flow cytometry for cell functions measurements in the fundamental and biomedical fields.

摘要

流式细胞术相对于传统技术具有重要优势。它速度快、灵敏度高，可进行多参数分析和细胞分选。流式细胞术有潜力用于测量多种细胞功能。该技术可用于确定细胞活力、细胞内钙和pH值、膜电位、酶活性、膜流动性和内吞作用。文中给出了许多关于流式细胞术在基础和生物医学领域用于细胞功能测量的应用实例。