Davies B R, Barraclough R, Davies M P, Rudland P S
Biochemistry Department, University of Liverpool.
Cell Biol Int. 1993 Sep;17(9):871-9. doi: 10.1006/cbir.1993.1150.
A syngeneic, immunocompetent rat mammary model has been employed to investigate the molecular basis of the metastatic phenotype. Transfection of the benign rat mammary epithelial cell line Rama 37 with the gene for p9Ka; a small, rat, calcium-binding protein or DNA from metastatic human cell lines derived from a primary breast carcinoma or a pleural effusion yields transfectants with metastatic capabilities. Transfection of DNA from a benign human mammary cell line, a plasmid containing a cDNA for the human basic fibroblast growth factor (bFGF) gene, or the oncogene EJ-ras-1, fails to yield any transfectants expressing the metastatic phenotype.
已采用同基因、具有免疫活性的大鼠乳腺模型来研究转移表型的分子基础。用p9Ka基因（一种小的大鼠钙结合蛋白）或源自原发性乳腺癌或胸腔积液的转移性人类细胞系的DNA转染良性大鼠乳腺上皮细胞系Rama 37，可产生具有转移能力的转染子。用来自良性人类乳腺细胞系的DNA、含有人类碱性成纤维细胞生长因子（bFGF）基因cDNA的质粒或癌基因EJ-ras-1进行转染，未能产生任何表达转移表型的转染子。
