Sugahara K, Kiyota T, Clark R A, Mason R J
Department of Anesthesiology, Kumamoto University School of Medicine, Japan.
Virchows Arch B Cell Pathol Incl Mol Pathol. 1993;64(2):115-22. doi: 10.1007/BF02915103.
The cytoskeleton of alveolar type II cells on different matrices has been examined, and the bioelectric properties of these cells grown as monolayers in primary culture has be measured using Ussing-type chambers, to determine whether the extracellular matrix affects the cytoskeletal organization of alveolar type II cells and whether any such interactions influence their physiological functions. Alveolar type II cells cultured on a fibronectin substratum spread slowly over a 6-day period to produce cells of extremely large diameter. The cytoskeletal structure of these cells was characterized by a more marked accumulation of large bundles of actin and a finer network of keratin than cells grown on a collagen substratum. The transepithelial resistances of monolayers grown on a fibronectin substratum were much higher than those on a collagen substratum. These results indicate that alveolar type II cells cultured on fibronectin can form tighter, better organized and more polarized monolayers in primary culture, which suggests that fibronectin may have a physiologically important role in the maintenance of the alveolar wall.
研究了不同基质上Ⅱ型肺泡细胞的细胞骨架，并使用尤斯灌流小室测量了原代培养中作为单层生长的这些细胞的生物电特性，以确定细胞外基质是否影响Ⅱ型肺泡细胞的细胞骨架组织，以及任何此类相互作用是否会影响其生理功能。在纤连蛋白基质上培养的Ⅱ型肺泡细胞在6天时间内缓慢铺展，形成直径极大的细胞。与在胶原基质上生长的细胞相比，这些细胞的细胞骨架结构的特征是肌动蛋白大束的积累更为明显，角蛋白网络更细密。在纤连蛋白基质上生长的单层细胞的跨上皮电阻远高于在胶原基质上的。这些结果表明，在纤连蛋白上培养的Ⅱ型肺泡细胞在原代培养中可以形成更紧密、组织更良好且更极化的单层，这表明纤连蛋白可能在维持肺泡壁方面具有重要的生理作用。
