Bishop G A, Chen Y F, Burry R W, King J S
Department of Cell Biology, Ohio State University, Columbus, OH 43210.
Brain Res. 1993 Oct 1;623(2):293-8. doi: 10.1016/0006-8993(93)91440-4.
An antibody to glutamic acid decarboxylase (GAD) was used to identify GABAergic terminals around the somata of basket cells in the cat's cerebellar cortex. Two sources for GAD immunoreactive terminals were identified based on size and cytological characteristics including the recurrent collaterals derived from Purkinje cells and the axons of stellate cells. The majority of the unlabeled terminals likely arise from parallel fibers. The present analysis indicates that GAD-positive terminals form 13% of the synaptic contacts on basket cells. The remaining 87% are unlabeled. Thus, GAD-positive terminals represent a small proportion of the synaptic input to basket cell bodies as compared to afferent endings that likely mediate excitation.
使用谷氨酸脱羧酶（GAD）抗体来识别猫小脑皮质中篮状细胞胞体周围的γ-氨基丁酸能终末。根据大小和细胞学特征确定了两种GAD免疫反应性终末来源，包括来自浦肯野细胞的返回侧支和星状细胞的轴突。大多数未标记的终末可能来自平行纤维。目前的分析表明，GAD阳性终末在篮状细胞上形成的突触联系占13%。其余87%未被标记。因此，与可能介导兴奋的传入终末相比，GAD阳性终末在篮状细胞胞体的突触输入中占比很小。
