溶液中单个单链DNA分子的荧光视频显微镜成像。
Imaging by fluorescence videomicroscopy of individual single stranded DNA molecules in solution.
作者信息
Auzanneau I, Barreau C, Salomé L
机构信息
Centre de Recherche, Paul Pascal, CNRS, Pessac, France.
出版信息
C R Acad Sci III. 1993;316(5):459-62.
PMID:8221228
Abstract
We demonstrate that the observation by fluorescence video-microscopy of individual single stranded DNA molecules is now possible owing to the use of an asymmetric cyanine (YOYO-1) belonging to a new class of fluorescent dyes complexing the DNA. The fluorescence of labelled DNA persists in denaturing conditions showing that the dye does not attach simply to the possible secondary structures. Furthermore it is stable under electrophoresis and enables the imaging of stretched molecules.
摘要
我们证明，由于使用了一种属于新型荧光染料的不对称花青染料（YOYO-1）与DNA复合，现在通过荧光视频显微镜观察单个单链DNA分子成为可能。标记DNA的荧光在变性条件下持续存在，表明该染料并非简单地附着于可能的二级结构。此外，它在电泳过程中稳定，能够对拉伸的分子进行成像。
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