金属硫蛋白与睾丸生殖细胞肿瘤及耐药性。临床相关性。
Metallothionein in testicular germ cell tumors and drug resistance. Clinical correlation.
作者信息
Chin J L, Banerjee D, Kadhim S A, Kontozoglou T E, Chauvin P J, Cherian M G
机构信息
Division of Urology, University of Western Ontario, London, Canada.
出版信息
Cancer. 1993 Nov 15;72(10):3029-35. doi: 10.1002/1097-0142(19931115)72:10<3029::aid-cncr2820721027>3.0.co;2-6.
BACKGROUND
Metallothioneins (MT) are endogenous metalloproteins involved in the homeostasis of essential metals and detoxification of toxic metals. Some recent experimental studies suggested tumor resistance to cisdiamminedichloroplatin may be associated with overexpression of MT in the tumor.
METHODS
The presence of MT in 33 primary testicular germ cell tumor specimens was assessed immunohistochemically using a rabbit polyclonal rat liver MT antibody that cross-reacted with human MT. The data were correlated with the patients' clinical course.
RESULTS
Seminomas stained weakly or not at all for MT, regardless of the clinical stage. Most nonseminomas stained heavily for MT. The more advanced staged nonseminomas tended to stain more heavily for MT.
CONCLUSIONS
In view of the considerable experimental evidence as well as some inferential clinical data involving MT in cis-diamminedichloroplatin resistance, there appears to be a role for MT in cis-diamminedichloroplatin resistance in germ cell tumors. Further studies to elucidate the role of MT in germ cell tumor chemoresistance are warranted.
背景
金属硫蛋白（MT）是一种内源性金属蛋白，参与必需金属的稳态调节以及有毒金属的解毒过程。最近的一些实验研究表明，肿瘤对顺二氯二氨铂的耐药性可能与肿瘤中MT的过表达有关。
方法
使用与人类MT发生交叉反应的兔抗大鼠肝脏MT多克隆抗体，通过免疫组织化学方法评估33例原发性睾丸生殖细胞肿瘤标本中MT的存在情况。将数据与患者的临床病程相关联。
结果
无论临床分期如何，精原细胞瘤对MT染色较弱或根本不染色。大多数非精原细胞瘤对MT染色强烈。分期越晚的非精原细胞瘤对MT染色往往越强。
结论
鉴于有大量实验证据以及一些涉及MT与顺二氯二氨铂耐药性相关的临床推断数据，MT在生殖细胞肿瘤对顺二氯二氨铂的耐药性中似乎起作用。有必要进一步研究以阐明MT在生殖细胞肿瘤化疗耐药性中的作用。
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