Yun K, Fidler A E, Eccles M R, Reeve A E
Department of Pathology, University of Otago Medical School, Dunedin, New Zealand.
Cancer Res. 1993 Nov 1;53(21):5166-71.
In situ hybridization was used to examine, in parallel, the localization of insulin-like growth factor II (Igf2) and WT1 transcripts in normal fetal kidney and Wilms' tumor. The expression of Igf2 and WT1 transcripts in the fetal kidney is almost complementary in both the epithelial and stromal cell lineages derived from the undifferentiated metanephrogenic blastema. The patterns of transcription of Igf2 in three Wilms' tumors appeared to be perturbed as compared to the normal fetal kidney. In these tumors Igf2 transcripts were detected in structures that are developmentally equivalent to the renal vesicle, which in the normal kidney do not contain Igf2 transcripts. These results suggest that Wilms' tumors arise from an alteration in the regulation of Igf2 mRNA synthesis.
采用原位杂交技术同时检测正常胎儿肾脏和肾母细胞瘤中胰岛素样生长因子II（Igf2）和WT1转录本的定位。在源自未分化后肾胚基的上皮和基质细胞谱系中，胎儿肾脏中Igf2和WT1转录本的表达几乎互补。与正常胎儿肾脏相比，三种肾母细胞瘤中Igf2的转录模式似乎受到干扰。在这些肿瘤中，在发育上等同于肾小囊的结构中检测到Igf2转录本，而在正常肾脏中肾小囊中不含有Igf2转录本。这些结果表明，肾母细胞瘤源于Igf2 mRNA合成调控的改变。
