Aldaz C M, Gollahon L S, Chen A
University of Texas M. D. Anderson Cancer Center, Science Park-Research Division, Smithville 78957.
Cancer Res. 1993 Nov 15;53(22):5339-44.
Progression of well differentiated rat mammary adenocarcinomas to very anaplastic phenotypes was found to correlate with a systematic and significant amplification of a mutant HRAS allele. Tumors with high amplification levels of this oncogene were analyzed by chromosomal in situ hybridization; in four of the cases the amplified sequences did not reside at the native chromosome 1 locus but were localized in a novel marker chromosome. The model described has potential as a reproducible system for the study of the chromosomal and cellular mechanisms operative "in vivo" for oncogene amplification.
