Davies B, Waxman J, Wasan H, Abel P, Williams G, Krausz T, Neal D, Thomas D, Hanby A, Balkwill F
Biological Therapies Laboratory, Imperial Cancer Research Fund, London, United Kingdom.
Cancer Res. 1993 Nov 15;53(22):5365-9.
We have used quantitative zymography to measure levels of the type IV collagenases matrix metalloproteinase (MMP)-9 and MMP-2 in 42 biopsies of transitional cell carcinoma and in 7 biopsies of normal bladder. Mean levels of MMP-9 were significantly higher in tumor compared with normal samples (P = 0.08). Levels of MMP-9 and active MMP-2 increased with tumor grade (test for trend, P = 0.002 and P = 0.05, respectively). Levels of MMP-9 and activated MMP-2 were also higher in invasive tumors than in superficial ones (P = 0.001 and P = 0.008, respectively). In situ hybridization studies showed that the mRNAs for both MMP-2 and MMP-9 were located chiefly in the stroma rather than epithelial tumor cells and were concentrated at the interface between the two tissues.
我们采用定量酶谱法检测了42例移行细胞癌活检组织和7例正常膀胱活检组织中IV型胶原酶基质金属蛋白酶（MMP）-9和MMP-2的水平。与正常样本相比，肿瘤组织中MMP-9的平均水平显著更高（P = 0.08）。MMP-9和活性MMP-2的水平随肿瘤分级升高而增加（趋势检验，P分别为0.002和0.05）。浸润性肿瘤中MMP-9和活化MMP-2的水平也高于浅表性肿瘤（P分别为0.001和0.008）。原位杂交研究表明，MMP-2和MMP-9的mRNA主要位于基质而非上皮肿瘤细胞中，且集中在两种组织的交界处。
