Ravingerová T, Tribulová N, Ziegelhöffer A, Styk J, Szekeres L
Institute for Heart Research, Slovak Academy of Sciences, Bratislava.
Cardiovasc Res. 1993 Jun;27(6):1051-5. doi: 10.1093/cvr/27.6.1051.
The aim was to investigate the late effect of pretreatment with 7-oxo prostacyclin on reperfusion induced arrhythmias in the isolated rat heart.
Forty eight hours after intramuscular administration of drug in vivo (50 micrograms.kg-1 body weight), isolated Langendorff perfused rat hearts were subjected to 30 minutes of regional ischaemia and 5 minutes of reperfusion. Incidence and duration of ventricular arrhythmias in both pretreated and control groups were evaluated on reperfusion. Morphological examination was also performed.
In the untreated group reperfusion induced 75% of sustained ventricular fibrillation. Incidence of ventricular fibrillation, its duration, and arrhythmia score were significantly lower in the pretreated group. Pretreatment with 7-oxo prostacyclin had no effect on heart rate and coronary flow throughout the whole course of perfusion. Neither was the occluded zone size affected. Ultrastructure of ischaemic and reperfused myocardium was better preserved in the pretreated group.
Antiarrhythmic action of 7-oxo prostacyclin was unrelated to changes in haemodynamics, thus suggesting the direct influence of the myocardium. The possible mechanism of action may involve maintenance of intracellular cation homeostasis (particularly of Na+ and Ca2+) due to a stimulation of sarcolemmal Na+ pump activity.
研究7-氧代前列环素预处理对离体大鼠心脏再灌注诱导的心律失常的晚期影响。
在体内肌肉注射药物（50微克·千克-1体重）48小时后，对离体Langendorff灌注大鼠心脏进行30分钟的局部缺血和5分钟的再灌注。在再灌注时评估预处理组和对照组室性心律失常的发生率和持续时间。还进行了形态学检查。
在未治疗组中，再灌注诱导了75%的持续性心室颤动。预处理组心室颤动的发生率、持续时间和心律失常评分显著较低。在整个灌注过程中，7-氧代前列环素预处理对心率和冠状动脉血流没有影响。闭塞区域大小也未受影响。预处理组缺血和再灌注心肌的超微结构保存得更好。
7-氧代前列环素的抗心律失常作用与血流动力学变化无关，因此提示对心肌有直接影响。可能的作用机制可能涉及由于刺激肌膜钠泵活性而维持细胞内阳离子稳态（特别是钠和钙）。
