Romero K M, Butcher B A, Boyle P J, Fry D E, Glew R H
Department of Biochemistry, School of Medicine, University of New Mexico, Albuquerque 87131.
Clin Chim Acta. 1993 Jul 16;216(1-2):125-33. doi: 10.1016/0009-8981(93)90145-t.
The activities of three lysosomal hydrolases and creatinine levels were measured in the plasma and urine of 11 adults (mean age, 28.1 years) with insulin-dependent diabetes mellitus and 14 non-diabetic controls (mean age, 27.9 years). All of the patients were free of diabetic complications and non exhibited microalbuminuria. Fractional enzyme excretion (FEE) values between the two groups of subjects were calculated and compared for the following enzymes: beta-hexosaminidase (N-acetyl-glucosaminidase), beta-glucuronidase and alpha-galactosidase. The FEE value was calculated as the ratio of enzyme clearance to creatinine clearance. Relative to the non-diabetic control group, the FEE value for beta-hexosaminidase was approximately 2-fold lower (P = 0.02) in the diabetic subjects (means, 0.424 vs. 0.242, respectively). The FEE values for beta-glucuronidase and alpha-galactosidase were not significantly different (P > 0.4) between the diabetic and control groups. These easily measured biochemical parameters in blood and urine and the resultant FEE value for beta-hexosaminidase may provide a means of assessing subtle deteriorative changes in renal function which occur in the early stage of diabetes before the onset of clinically evident complications.
在11名胰岛素依赖型糖尿病成年患者（平均年龄28.1岁）和14名非糖尿病对照者（平均年龄27.9岁）的血浆和尿液中，测量了三种溶酶体水解酶的活性以及肌酐水平。所有患者均无糖尿病并发症，且均未出现微量白蛋白尿。计算并比较了两组受试者之间以下几种酶的分数排泄率（FEE）值：β-己糖胺酶（N-乙酰葡糖胺酶）、β-葡萄糖醛酸酶和α-半乳糖苷酶。FEE值的计算方法为酶清除率与肌酐清除率之比。与非糖尿病对照组相比，糖尿病受试者中β-己糖胺酶的FEE值约低2倍（P = 0.02）（平均值分别为0.424和0.242）。糖尿病组和对照组之间β-葡萄糖醛酸酶和α-半乳糖苷酶的FEE值无显著差异（P > 0.4）。这些血液和尿液中易于测量的生化参数以及由此得出的β-己糖胺酶的FEE值，可能提供一种评估糖尿病早期在临床明显并发症出现之前肾功能细微恶化变化的方法。
